Abdominal Aortic Calcium and Adipokines

A random subset of all MESA participants (n = 1,970) underwent abdominal computed tomography (CT) scans at either exam 2 or exam 3 (randomly assigned) to measure abdominal aortic calcium (40 (link)). In a subsequent ancillary study related to body composition, adipokine levels were measured among these same participants from stored frozen serum samples obtained at the respective exams (2 or 3) of their CT scan (30 (link)). The adipokines (adiponectin, leptin, and resistin) were analyzed using a Bio-Rad Luminex flow cytometry (Millepore, Billerica, MA) at the Laboratory for Clinical Biochemistry Research (University of Vermont, Burlington, VT), as previously reported (31 (link), 32 (link)). The coefficients of variation for these assays ranged from 6 to 13%.

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Varma B., Ogunmoroti O., Ndumele C.E., Kazzi B., Rodriquez C.P., Osibogun O., Allison M.A., Bertoni A.G, & Michos E.D. (2023). Associations between endogenous sex hormone levels and adipokine levels in the Multi-Ethnic Study of Atherosclerosis. Frontiers in Cardiovascular Medicine, 9, 1062460.

Publication 2022

Luminex flow cytometry

Abdominal Abdominal aortic Adipokines Adiponectin Assays Body composition Calcium Computed tomography Flow cytometry Frozen Leptin Resistin Scan Serum

Corresponding Organization : Johns Hopkins University

Other organizations : Florida International University, University of California, San Diego, Wake Forest University

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Variable analysis

independent variables

Abdominal computed tomography (CT) scans at either exam 2 or exam 3 (randomly assigned)

dependent variables

Abdominal aortic calcium
Adipokine levels (adiponectin, leptin, and resistin)

control variables

Stored frozen serum samples obtained at the respective exams (2 or 3) of their CT scan

controls

No positive or negative controls were explicitly mentioned in the provided information.

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