Canine Hemangiosarcoma Cell Lines

Samples used to derive canine hemangiosarcoma cell lines from 10 pet dogs [19] (link), [23] (link), [24] (link) are listed in Table 1. Only two of the dogs whose samples were used for the microarray experiments (Frog and Journey) were related within 5 generations, and they were separated by 3 generations (Frog was Journey's “great aunt”), reducing the likelihood of lineage bias. Cryopreserved cultured cells from the earliest available passages were used for these experiments. Peripheral blood samples collected from healthy dogs or from dogs with cancer prior to the initiation of any therapy (at the time of tumor biopsy) were used as controls. Non-hemangiosarcoma diagnoses included non-Hodgkin lymphoma, melanoma, and osteosarcoma. Blood samples were age and sex-matched to reduce variation. Every sample used for this study was obtained with owner consent through protocols reviewed by appropriate Institutional Animal Care and Use Committees. Samples from healthy pet dogs were obtained as part of routine diagnostic or well-health procedures. Samples from pet dogs with cancer were obtained by the attending veterinarian as part of medically necessary (biopsy) procedures or at the time of necropsy.