Protocol detail

Automated DNA Extraction and Microsatellite Genotyping

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Genomic DNA was extracted using the QIAxtractor and QIAcube robots (Qiagen) following the manufacturer's instructions. Samples were screened at 30 microsatellite markers from Peyran et al. (2020 (link)) and González‐Wangüemert et al. (2014 (link)) following the same genotyping procedure as described in Peyran, Boissin, et al. (2021 (link)). Allele sizes were scored and checked manually using GeneMapper v.5 software (Applied Biosystems). Samples presenting ambiguous peak profiles were re‐amplified, genotyped, and re‐scored, and all peaks that remained unclear were considered as missing data.

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Peyran C., Boissin E., Morage T., Nebot‐Colomer E., Iwankow G, & Planes S. (2022). Investigating population dynamics from parentage analysis in the highly endangered fan mussel Pinna nobilis. Ecology and Evolution, 12(1), e8482.

Publication 2022

QIAxtractor QIAcube GeneMapper v.5

Allele Genomic Microsatellite markers

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Variable analysis

independent variables

Not explicitly mentioned.

dependent variables

Not explicitly mentioned.

control variables

Genomic DNA was extracted using the QIAxtractor and QIAcube robots (Qiagen) following the manufacturer's instructions.
Samples were screened at 30 microsatellite markers from Peyran et al. (2020) and González‐Wangüemert et al. (2014) following the same genotyping procedure as described in Peyran, Boissin, et al. (2021).
Allele sizes were scored and checked manually using GeneMapper v.5 software (Applied Biosystems).
Samples presenting ambiguous peak profiles were re‐amplified, genotyped, and re‐scored, and all peaks that remained unclear were considered as missing data.

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