Chronic Thromboembolic Pulmonary Hypertension Model

CTEPH was induced in animals by means of repetitive embolization of distal branches of pulmonary artery with partially biodegradable alginate microspheres (MS) of 160–200 μm in diameter. MSs were produced from ultrapure alginate natrium (Sigma-Aldrich, St. Louis, MO, USA) using 2% barium chloride as a stabilizer with electrostatic encapsulator (B-390, Buchi, Flawil, Switzerland). All MSs were produced in sterile conditions. 50 μL of MSs by volume were suspended in 1 mL of 0.9% NaCl solution and administered via the tail vein 8 times separated by 4-day intervals [26 (link),27 (link)]. Ten rats received injections of 0.9% NaCl solution instead of MSs suspension, thus forming the group of intact animals (INT).

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Karpov A.A., Vachrushev N.S., Shilenko L.A., Smirnov S.S., Bunenkov N.S., Butskih M.G., Chervaev A.K., Vaulina D.D., Ivkin D.Y., Moiseeva O.M, & Galagudza M.M. (2023). Sympathetic Denervation and Pharmacological Stimulation of Parasympathetic Nervous System Prevent Pulmonary Vascular Bed Remodeling in Rat Model of Chronic Thromboembolic Pulmonary Hypertension. Journal of Cardiovascular Development and Disease, 10(2), 40.

Publication 2023

B-390

0 9 nacl Alginate Animals Barium chloride Electrostatic Embolization Microspheres Pulmonary artery Rats Sterile Tail Vein

Corresponding Organization :

Other organizations : Ministry of Health of the Russian Federation, Institute of Experimental Medicine, Federal Almazov North-West Medical Research Centre, First Pavlov State Medical University of St. Petersburg

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Variable analysis

independent variables

Repeated administration of alginate microspheres (MS) via tail vein injection

dependent variables

Development of chronic thromboembolic pulmonary hypertension (CTEPH) in animals

control variables

Administration of 0.9% NaCl solution instead of MS suspension in the group of intact animals (INT)

controls

Positive control: Animals receiving repeated injections of alginate microspheres to induce CTEPH
Negative control: Intact animals receiving 0.9% NaCl solution instead of MS suspension

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