Large preparations of VNI, FF-VNI, VFV, and F-VFV were synthesized by the Chemical Synthesis Core Facility (Vanderbilt Institute of Chemical Biology),34 (link) and synthesis of their analogues CF3F-VNI and VFV-Cl has been described previously.9 (link),10 (link) The purity of the compounds was >95%. Voriconazole, ketoconazole, itraconazole, and posaconazole were purchased from Santa Cruz Biotechnology (Dallas, TX); isavuconazole was from Sigma-Aldrich, and fluconazole was from ICN Biomedicals. Hydroxypropyl-β-cyclodextrin (HPCD) was purchased from Cyclodextrin Technology Development (Gainesville, FL). DEAE- and CM-Sepharose resins were from GE Healthcare, and Ni2+-nitrilotriacetate (NTA) agarose was purchased from Qiagen.
Protocol detail
Synthesis and Characterization of Novel Antifungal Compounds
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Voriconazole
ketoconazole
itraconazole
posaconazole
isavuconazole
fluconazole
Ni2+-nitrilotriacetate (NTA) agarose
Agarose
Cyclodextrin
Deae
Fluconazole
Hydroxypropyl
Isavuconazole
Itraconazole
Ketoconazole
Posaconazole
Resins
Synthesis
Voriconazole
Corresponding Organization : Vanderbilt University
Other organizations : Northwestern University, Meharry Medical College, Texas Tech University
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Variable analysis
independent variables
- Preparations of VNI, FF-VNI, VFV, and F-VFV
- Analogues CF3F-VNI and VFV-Cl
- Purity of the compounds
- Voriconazole, ketoconazole, itraconazole, posaconazole, isavuconazole, fluconazole
- Hydroxypropyl-β-cyclodextrin (HPCD)
- DEAE- and CM-Sepharose resins
- Ni2+-nitrilotriacetate (NTA) agarose
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