Measuring WRN-dependent DNA repair efficiency
Corresponding Organization :
Other organizations : Istituto Superiore di Sanità, Università degli Studi della Tuscia, National Institute on Aging
Variable analysis
- PCMV-FlagRnai-resWRN^WT
- PCMV-FlagRnai-resWRN^S1133A
- PCMV-FlagRnai-resWRN^S1133D
- I-SceI expression vector pCBASceI
- PHPRT-DRGFP plasmid reporter
- I-SceI linearized NHEJ reporter
- Percentage of GFP-positive cells
- Percentage of repair efficiency
- Cell density (0.5 million cells per well)
- Cell line (HEK293TshWRN)
- Transfection reagent (DreamFect)
- Incubation time (72 hours)
- Positive control: Cells transfected with wild-type WRN (pCMV-FlagRnai-resWRN^WT)
- Negative control: Cells without I-SceI transfection
Annotations
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