Corneal Injury Induces CD11c+ Cells

We have previously reported that 2.0-mm circular incisions of the corneal epithelium induce the generation of CS cells that express the CD11c surface marker.²⁰ (link) Corneas of BALB/c mice were trephined as described previously, and CD11c⁺ spleen cells were isolated 4 days later using a Miltenyl Biotec (Santa Barbara, CA) pan dendritic cell (DC) isolation kit.²⁰ (link) The CD11c⁺ cells from trephined mice and untreated mice were used for detecting regulatory cell activity in vitro.²⁰ (link)

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Neelam S, & Niederkorn J.Y. (2020). Corneal Nerve Ablation Abolishes Ocular Immune Privilege by Downregulating CD103 on T Regulatory Cells. Investigative Ophthalmology & Visual Science, 61(4), 25.

Publication 2020

pan dendritic cell (DC) isolation kit

Balb c mice Cell Cell isolation Corneal epithelium Corneas Dendritic Dendritic cell Isolation Mice Spleen

Corresponding Organization : The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

Corneal epithelial incisions (2.0-mm circular)

dependent variables

Generation of CD11c+ corneal stromal (CS) cells

control variables

BALB/c mice

controls

Positive control: CD11c+ spleen cells isolated from trephined (incised) mice
Negative control: CD11c+ spleen cells isolated from untreated (non-incised) mice

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