Quantifying Antioxidant Capacity via Folin-Ciocalteu Assay
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Corresponding Organization :
Other organizations : University of Arkansas at Pine Bluff, University of Arkansas at Little Rock
Protocol cited in 32 other protocols
Variable analysis
- Concentration of test compound
- Absorbance at 765 nm
- Volume of water (1.58 mL)
- Volume of F-C reagent (0.1 mL)
- Volume of test compound dilution (20 μL)
- Volume of 20% aqueous sodium carbonate solution (0.3 mL)
- Incubation time (5 minutes before addition of sodium carbonate, and 30 minutes at 45°C after addition of sodium carbonate)
- Spectrophotometer used (Bio-Rad Smart Spec 3000)
- Gallic acid standard curve
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