Targeted Silencing of NEAT1 and LIN28B

The NEAT1-shRNA-Lentivirus for use in the in vivo experiments was purchased from the ABM Company. LIN28B-related stable cell lines were constructed according to previously published guidelines^{51 (link)}. siRNAs targeting NEAT1 and LIN28B were purchased from Suzhou Synbio Technologies and transfected into cells using Lipofectamine 3000 (Invitrogen). All siRNA and shRNA sequences are provided in Supplementary Table 5. miR-506 inhibitor was purchased from GenePharma (Shanghai, China).

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Yong W., Yu D., Jun Z., Yachen D., Weiwei W., Midie X., Xingzhu J, & Xiaohua W. (2018). Long noncoding RNA NEAT1, regulated by LIN28B, promotes cell proliferation and migration through sponging miR-506 in high-grade serous ovarian cancer. Cell Death & Disease, 9(9), 861.

Publication 2018

Lipofectamine 3000 miR-506 inhibitor

Cell lines Cells Lentivirus Lipofectamine Shrna Sirna

Corresponding Organization :

Other organizations : Fudan University Shanghai Cancer Center, Shanghai Medical College of Fudan University

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Variable analysis

independent variables

NEAT1 knockdown using NEAT1-shRNA-Lentivirus
LIN28B knockdown using siRNAs targeting LIN28B
MiR-506 inhibition using a miR-506 inhibitor

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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