Cells were isolated as previously described (18 (link)). After dissection, the pancreas was digested using collagenase-containing solution (200 IU/ml, Worthington) and incubated in a 37°C water bath for 14 to 15 minutes. The extracellular solution contained the following: 140 mM NaCl, 4.7 mM KCl, 10 mM HEPES, 1 mM MgCl2, 10 mM glucose, pH 7.3, and 1 mM CaCl2. Osmolarity was checked by Osmomat 030. All in vitro experiments were conducted using this solution unless otherwise stated.
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