Deacylation reactions were performed as described for SIRT3 [10 (link)] and SIRT5 [7 (link)]. In some experiments deacylation was followed as the cleavage of 14C-NAD+ (Perkin Elmer, Waltham, MA) to 14C-NAM as described [10 (link)] with a reaction time of 20 minutes at 37°C.
Free full text: Click here