RNA oligonucleotides of unedited miR-379, edited miR-379, miR-380, unedited miR-411, edited miR-411, and miR-758 were purchased from Integrated DNA Technologies, dissolved in IDTE buffer, pH 7.5 (Integrated DNA Technologies), and then diluted in 10-fold dilution series for RT-qPCR. For experiments with yeast RNA background, 100 ng total yeast RNA (#AM7118, Invitrogen, Thermo Scientific) was added during RT sample preparation. DNA oligonucleotides were purchased from Invitrogen. Two-tailed RT primers were designed based on a hairpin sequence published by Androvic et al. (2017) (link) with hemiprobes designed to bind unedited or edited miR-379, and primer arms optimized to prevent the formation of unwanted secondary structures. Secondary structures of RT primers as well as secondary structures and dimers for qPCR primers were calculated using the OligoAnalyzer tool (Integrated DNA Technologies). ZEN/Iowa Black FQ double-quenched FAM-coupled miR-379 hydrolysis probe was designed using the PrimerQuest tool (Integrated DNA Technologies) and purchased from Integrated DNA Technologies. Primers for pri-miR-379 RT-PCR were based on those published by Kawahara et al. (2008) (link), and primers for cloning were designed using the NEBuilder tool (New England Biolabs). All RNA and DNA oligonucleotide sequences are listed in Supplemental Tables 2 and 3.