Carbohydrate Metabolism and Growth Profiling of Sourdough LAB

The metabolism of several carbohydrates by sourdough LAB strains was determined by using API 50 CHL galleries (BioMérieux, Marcy-l’Etoile, France) according to the manufacturer instructions. Gas production was detected by Durham tube method [37 ] in MRS broth (Biolife, Milan, Italy) for 24 h at 30 °C. The growth performance of strains was monitored at 10, 30, 37 and 45 °C for 24 h in an MRS broth using a Thermo Bioscreen C automatic turbidometer (Labsystems, Helsinki, Finland). The viability of the isolated strains to grow in acidic environments was evaluated in MRS broth acidified to a final pH of 2.5 with HCl (Biolife, Milan, Italy) in tubes, according to Lee et al. [38 (link)]. Total viable counts were determined by using standard plate count techniques [39 ]. The results were expressed as log of colony-forming units (CFU) per milliliter. All phenotype analyses were carried out in triplicate.

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Bartkiene E., Lele V., Ruzauskas M., Domig K.J., Starkute V., Zavistanaviciute P., Bartkevics V., Pugajeva I., Klupsaite D., Juodeikiene G., Mickiene R, & Rocha J.M. (2019). Lactic Acid Bacteria Isolation from Spontaneous Sourdough and Their Characterization Including Antimicrobial and Antifungal Properties Evaluation. Microorganisms, 8(1), 64.

Publication 2019

API 50 CHL galleries MRS broth

Acidic Carbohydrates metabolism Phenotype Strains

Corresponding Organization : Lithuanian University of Health Sciences

Other organizations : BOKU University, University of Latvia, Institute of Food Safety, Animal Health and Environment “BIOR”, Kaunas University of Technology, Vytautas Magnus University, Rede de Química e Tecnologia, Universidade do Porto

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Variable analysis

independent variables

Carbohydrate metabolism by sourdough LAB strains
Growth performance of strains at 10, 30, 37 and 45 °C
Viability of isolated strains to grow in acidic environments (pH 2.5)

dependent variables

Gas production detected by Durham tube method
Growth performance monitored using Thermo Bioscreen C automatic turbidometer
Total viable counts determined by standard plate count techniques

control variables

MRS broth (Biolife, Milan, Italy) used for growth and gas production experiments
Incubation temperature and duration (24 h) for growth experiments
MRS broth acidified to pH 2.5 with HCl (Biolife, Milan, Italy) used for viability experiments

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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