BCD granule stability and quality were evaluated utilizing a high-performance liquid chromatography (HPLC) system (Agilent 1100, USA) equipped with a Waters Xbridge-C18 analytical column (4.6 × 150 mm, 5 μm). Mobile phase A indicated the acetonitrile organic phase, while mobile phase B indicated the ultrapure water (containing 0.1% phosphoric acid) aqueous phase. Mobile phases were altered based on the following: 0–5 min, 10% (phase A); 5–35 min, 10%–20% (phase A); 35–50 min, 20%–30% (phase A); 50–55 min, 30%–37% (phase A), 55–60 min, 37%–10% (phase A). The flow rate was 1.0 ml/min and the UV spectrum was calibrated to 230 nm. Specnuezhenide (CAS: 39011-92-2), cyasterone (CAS: 17086-76-9), ferulic acid (CAS: 537-98-4) and isorhamnetin-3-O-neohesperidoside (CAS: 55033-90-4) were procured from National Institutes for Food and Drug Control (Beijing, China), and hyperoside (CAS: 482-36-0), asperosaponin VI (CAS: 39524-08-8), peoniflorin (CAS: 23180-57-6) and typhaneoside (CAS: 104472-68-6) were obtained from Shanghai Standard Technology Co., Ltd. (Shanghai, China). The detection wavelength of BCD was identified based on retention time compared with reference substance.
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