Long-term Effects of IL18 on Food Intake, Body Composition, and iWAT

To assess the long-term central effects of the IL18 system on food intake, body composition and iWAT morphology, 10–11 weeks old Il18r1-KO and WT mice (4–7 mice/genotype/treatment) were infused ICV with saline or recombinant IL18 (20 μg/mouse/7 days, Sino Biological, Inc., Beijing, China)56 (link). For this purpose, brain infusion canulae were placed stereotaxically into the lateral ventricle as previously described56 (link). A catheter tube was connected from the brain infusion cannula to the osmotic minipump (model 1007D, total volume 100 μL, flow rate 0.5 μL/h; Alzet Osmotic Pumps, Durect, CA, USA) flow moderator.