Microscopic Imaging of Fungal Hyphae

For microcopy, an Olympus System microscope Model BX51 (Olympus) equipped with UPlanSApo 60X and UPlanFL 100X objective lenses (Olympus) and stereomicroscope Model SMZ800 (Nikon) were used. Images were captured with a DP71 digital camera (Olympus) and processed using the DP manager imaging software (Olympus). For microscopic observation of the fungal hyphae, each strain was coverslip-cultured on a block of appropriate agar medium or incubated in liquid GMM medium. The coverslips were stained with 1 mg/ml Hoechst 33342 (Sigma-Aldrich) for labeling DNA^{2 (link)}. DAPI (high brightness) filter cubes (excitation filter: center wavelength 377 nm, emission filter: center wavelength 447 nm, Olympus) and FITC filter cubes (excitation filter: center wavelength 483 nm, emission filter: center wavelength 535 nm, Olympus) were used to observe the fluorescence of Hoechst and YFP, respectively^{50 (link)}.