The putative PHB accumulation by the strain 2D1 grew in MSM culture with glucose was weighed and recorded. The amount of putative PHB extracted from the strain 2D1 pellet was calculated [27 (link)], according to the following Equation (2):
The estimations of the DCW, residual biomass, and bio-based polymer accumulation were also quantified in the presence of fructose, maltose, saccharose, sorbitol, lactose, xylose, and mannose incorporated one at a time in the MSM to replace glucose (Fisher Scientific, Goteborg, Sweden) [29 (link)].
Additional measurements of DCW, residual biomass, and PHB production were repeated by using a pretreated argan pulp, i.e., a residue obtained from the extraction of oil from the argan seeds [25 (link)].
The estimations of the DCW, residual biomass, and bio-based polymer accumulation were also quantified in the presence of fructose, maltose, saccharose, sorbitol, lactose, xylose, and mannose incorporated one at a time in the MSM to replace glucose (Fisher Scientific, Goteborg, Sweden) [29 (link)].
Additional measurements of DCW, residual biomass, and PHB production were repeated by using a pretreated argan pulp, i.e., a residue obtained from the extraction of oil from the argan seeds [25 (link)].
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