Polyphenols were extracted from 3 g of lyophilized calli following the methods detailed in [15 (link)]. The ultrasonic calli extraction procedure was repeated three times. Polyphenols were quantified and separated using an UHPLC system equipped with a quaternary pump (Ultimate 3000, Thermo Fisher Scientific, Waltham, MA, USA), and a Kinetex 2.6 µm Biphenyl (100 × 2.1 mm, Phenomenex, Torrance, CA, USA) column. Mass spectrometry analysis was carried out with a Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific, Waltham, MA, USA).
The acquisition of polyphenolic compounds was performed according to [15 (link)], where the protocol is fully detailed. The calibration and accuracy of the equipment were monitored by using a reference standard mixture (Thermo Fisher Scientific, Waltham, MA, USA). Data acquisition and processing were performed with Quan/Qual Browser Xcalibur software, v. 3.1.66.10 (Xcalibur, Thermo Fisher Scientific, Waltham, MA, USA).
The acquisition of polyphenolic compounds was performed according to [15 (link)], where the protocol is fully detailed. The calibration and accuracy of the equipment were monitored by using a reference standard mixture (Thermo Fisher Scientific, Waltham, MA, USA). Data acquisition and processing were performed with Quan/Qual Browser Xcalibur software, v. 3.1.66.10 (Xcalibur, Thermo Fisher Scientific, Waltham, MA, USA).
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