Molybdenum and Cadmium Analysis in Rice

The aromatic rice plant leaves were sampled at three prominent growth stages and ears (at reproductive stage) and grains (at maturity stage) and oven dried for estimating Mo and Cd concentrations. The plant samples (0.3 g) were digested in 5:1 (v/v) HNO₃:HClO₄ (5 mL) in a microwave oven (MLS 1200, Milestone, FKV, Italy). Mo and Cd concentrations were measured by ICP-mass spectrometry (ICP-MS) (ELAN DRC-e, PerkinElmer SCIEX, DE) and Atomic Absorption Spectrophotometer (AAS) (AA6300C, Shimadzu, Japan) [2 (link),27 (link)].

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Imran M., Hussain S., He L., Ashraf M.F., Ihtisham M., Warraich E.A, & Tang X. (2021). Molybdenum-Induced Regulation of Antioxidant Defense-Mitigated Cadmium Stress in Aromatic Rice and Improved Crop Growth, Yield, and Quality Traits. Antioxidants, 10(6), 838.

Publication 2021

MLS 1200 AA6300C

Ears Grains Mass spectrometry Microwave Plant Plant leaves Reproductive Rice

Corresponding Organization : South China Agricultural University

Other organizations : University of Agriculture Faisalabad, Sichuan Agricultural University

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Variable analysis

independent variables

Growth stages of the aromatic rice plant (three prominent stages)
Parts of the rice plant (leaves, ears, grains)

dependent variables

Molybdenum (Mo) concentrations
Cadmium (Cd) concentrations

control variables

Mass of plant samples (0.3 g)
Digestion method (5:1 (v/v) HNO3:HClO4, 5 mL, microwave oven)
Analytical methods (ICP-MS, AAS)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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