Epigenetic Mouse Age Estimation

Genomic DNA was isolated from liver tissue samples from WT and HET mice using Quick DNA universal kit (Zymo Research). Evaluation of the biological age of these samples was done using the Mouse DNAge Epigenetic Aging Clock service offered by Zymo Research. This is a mouse biological age predictor based on DNA methylation levels of a small set of CpG sites [27 (link)]. This method is based on Hovrath’s aging clock [28 (link)] and uses the Simplified Whole-panel Amplification Reaction Method.

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Pathak S., Stewart W.C., Burd C.E., Hester M.E, & Greenberg D.A. (2020). Brd2 haploinsufficiency extends lifespan and healthspan in C57B6/J mice. PLoS ONE, 15(6), e0234910.

Publication 2020

Quick DNA universal kit

Biological Dna methylation Genomic Liver Mouse Tissue

Corresponding Organization : The Ohio State University

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Variable analysis

independent variables

Genotype (WT and HET mice)

dependent variables

Biological age of the samples

control variables

Liver tissue samples

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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