As previously described [20 (link)], cells were harvested in RIPA buffer (Sigma Aldrich). Whole cell lysate was separated using SDS-PAGE. Primary antibodies against FHL1 (Santa Cruz Biotechnology, sc-374246, 1:1000 diluted), Cyclin D1, Cyclin E, p53, p27, p21, phosph-ERK1/2, and ERK1/2 (Cell signaling Technology) were used in this study. β-actin antibody was used to normalize protein loading.
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