Western Blot Analysis of Cell Signaling

Western blotting was performed as described previously [20 (link),21 (link)]. The following antibodies were used in this study: anti-SMAD4 (sc-7154 or sc-7966), anti-E-cadherin (sc-8426), anti-vimentin (sc-7557), anti-CD133 (sc-8304), anti-CD44 (sc-18849), anti-Sp1(sc-14027), anti-c-Jun (sc-1694), anti-Fos (sc-52), anti-Fast-1 (sc-377358), anti-Hes1 (sc-25392), anti-GAPDH (sc-32233; Santa Cruz Biotechnology, Inc.), anti-p-Akt (#4060), anti-Akt (#4691), anti-p-p44/42 (#9101),anti-p44/42 (#4695), anti-Pten (#9272), anti-NF-κB (#4764S), anti- EGFR (#4267), anti-p-EGFR tyr 992 (#2235), anti-p-EGFR tyr 1068 (#3777), anti-Smad2/3 (#5339), anti-p-Smad2/3 (#3101), anti-p-c-Jun (#2361; Cell Signaling Technology, Inc.), anti-Nestin (N5413), mouse anti-β-actin (Sigma- Aldrich Co.), anti-CD133/1 (AC133, Miltenyi Biotec.) and anti-TGF-β1 (ab9758, Abcam, Plc.).

Free full text: Click here

Chen Y.W., Hsiao P.J., Weng C.C., Kuo K.K., Kuo T.L., Wu D.C., Hung W.C, & Cheng K.H. (2014). SMAD4 Loss triggers the phenotypic changes of pancreatic ductal adenocarcinoma cells. BMC Cancer, 14, 181.

Publication 2014

Actin Antibodies Cadherin Cd44 Egfr Gapdh Mouse Nestin Nf κb Pten Smad2 Smad4 Tgf β1 Vimentin

Corresponding Organization :

Other organizations : National Sun Yat-sen University, Kaohsiung Medical University, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung Municipal Hsiao-Kang Hospital, National Health Research Institutes

Top 5 similar protocols

Variable analysis

independent variables

Anti-SMAD4 (sc-7154 or sc-7966)
Anti-E-cadherin (sc-8426)
Anti-vimentin (sc-7557)
Anti-CD133 (sc-8304)
Anti-CD44 (sc-18849)
Anti-Sp1(sc-14027)
Anti-c-Jun (sc-1694)
Anti-Fos (sc-52)
Anti-Fast-1 (sc-377358)
Anti-Hes1 (sc-25392)
Anti-p-Akt (#4060)
Anti-Akt (#4691)
Anti-p-p44/42 (#9101)
Anti-p44/42 (#4695)
Anti-Pten (#9272)
Anti-NF-κB (#4764S)
Anti-EGFR (#4267)
Anti-p-EGFR tyr 992 (#2235)
Anti-p-EGFR tyr 1068 (#3777)
Anti-Smad2/3 (#5339)
Anti-p-Smad2/3 (#3101)
Anti-p-c-Jun (#2361)
Anti-Nestin (N5413)
Anti-CD133/1 (AC133, Miltenyi Biotec.)
Anti-TGF-β1 (ab9758, Abcam, Plc.)

dependent variables

SMAD4
E-cadherin
Vimentin
CD133
C-Jun
Fast-1
P-Akt
P-p44/42
P44/42
NF-κB
P-EGFR tyr 992
P-EGFR tyr 1068
Smad2/3
P-Smad2/3
P-c-Jun
Nestin
CD133/1
TGF-β1

control variables

GAPDH
Mouse anti-β-actin (Sigma-Aldrich Co.)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!