Primary mouse macrophages (BMDMs) were cultured in Iscove’s (IMDM media + 10% fetal bovine serum (FBS)). Cells were treated with CFTR modulators for 24 h at the following concentrations: 10 µM of tezacaftor (VX-661) (S7059, Selleckchem, Houston, TX, USA) and 5 µM of ivacaftor (VX-770) (S1144, Selleckchem). In vitro infections were performed as previously described (Krause et al., 2019 (link); Estfanous et al., 2021 (link)).
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