Quantification of Nitrate Levels

The nitrate content in the cultures were determined in the supernatant after centrifugation of the samples. The exact method was published elsewhere [36 (link)]. A Dionex ICS-6000 ion chromatography system (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an IonPac AS11 and guard column (Thermo Fisher Scientific, Waltham, MA, USA) was used. Detection was done via a conductivity detector unit (Thermo Fisher Scientific, Waltham, MA, USA). Quantification was possible via calibration of nitrate standards (Sigma-Aldrich, St. Louis, MO, USA).

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Doppler P., Kriechbaum R., Käfer M., Kopp J., Remias D, & Spadiut O. (2022). Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity. Marine Drugs, 20(3), 175.

Publication 2022

Dionex ICS-6000 IonPac AS11 nitrate standards

Centrifugation Chromatography Conductivity Nitrate

Corresponding Organization : TU Wien

Other organizations : University of Applied Sciences Upper Austria

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Variable analysis

independent variables

Nitrate content in the cultures

dependent variables

Nitrate content in the supernatant after centrifugation

control variables

Centrifugation process
Ion chromatography system (Dionex ICS-6000)
IonPac AS11 and guard column
Conductivity detector unit
Nitrate standards (Sigma-Aldrich)

controls

Positive control: Nitrate standards (Sigma-Aldrich)
Negative control: Not explicitly mentioned

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