Antemortem CSF was collected through standardized operating procedures as described8 (link). One of two analytical platforms (i.e. Innotest ELISA; Fujirebio-Europe or INNO-BIA AlzBio 3 xMAP Luminex; Fujirebio-Europe) were used to measure CSF t-tau, p-tau (threonine181) and Aβ1–42 as described8 (link). We previously found that absolute values for these analytes are highly correlated between platforms and can be transformed into equivalent units for analysis.8 (link), 27 (link), 28 (link) Thus, we used a validated algorithm8 (link) to transform ELISA t-tau and p-tau into equivalent xMAP units for analysis.
Protocol detail
Standardized CSF Biomarker Measurement
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Elisa
Corresponding Organization : University of Pennsylvania
Other organizations : Centro de Investigación Biomédica en Red, Hospital de Sant Pau, Biomedical Research Networking Center on Neurodegenerative Diseases
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Variable analysis
independent variables
- Analytical platform (Innotest ELISA or INNO-BIA AlzBio 3 xMAP Luminex)
- CSF t-tau
- CSF p-tau (threonine181)
- CSF Aβ1–42
- Standardized operating procedures for antemortem CSF collection as described in the referenced publication8
- Transformation of ELISA t-tau and p-tau into equivalent xMAP units using a validated algorithm8
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