Gomisin G, D, J, N and O were purchased from Biopurify Phytochemicals Ltd (Sichuan, China). The purity of the Gomisins were measured using an Agilent 1100 series high performance liquid chromatography (HPLC) system fitted with a RP-C18 column (Gemini, 5 μm, 4.6×250 mm; Phenomenex, Torrance, CA, USA) at room temperature as previously described (Maharjan et al., 2018 (link)). A UV/VIS detector (Agilent Technologies, Santa Clara, CA, USA) was used to measure the absorbance at 220 nm. The mobile phase was 65% aqueous acetonitrile, and the flow rate was 3.0 mL/min. Its chromatogram showed 98% purity; thus, it was used without further purification.
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Quantitative Analysis of Gomisins
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Corresponding Organization : Hallym University
Other organizations : Konkuk University, Chungbuk National University
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- Gomisin G, D, J, N and O
- Purity of Gomisins
- RP-C18 column (Gemini, 5 μm, 4.6×250 mm; Phenomenex, Torrance, CA, USA) at room temperature
- UV/VIS detector (Agilent Technologies, Santa Clara, CA, USA) used to measure the absorbance at 220 nm
- Mobile phase was 65% aqueous acetonitrile
- Flow rate was 3.0 mL/min
- Chromatogram showed 98% purity, so Gomisins were used without further purification
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