Blood was collected via a retro-orbital bleeding technique from anti-B7x treated mice and IgG-treated control mice following an 18-h fast. Serum levels of insulin were measured by ELISA with the Ultrasensitive Mouse Insulin ELISA Kit (Mercodia, NC, USA) as described previously (Shen et al., 2009 (link)). The expression levels of IFN-γ and TNF-α in tumor tissues from B7x treated mice and IgG control mice were measured by a mouse IFN-γ ELISA kit (Abcam, MA, USA) and a mouse TNF-α ELISA kit (Life Technologies, MD, USA) following the manufacturer protocols.
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Serum Insulin and Cytokine Levels in Anti-B7x Mice
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Corresponding Organization : Rutgers, The State University of New Jersey
Other organizations : Johnson University, Albert Einstein College of Medicine, Yeshiva University, University of Iowa
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Variable analysis
independent variables
- Anti-B7x treatment
- IgG treatment
- Serum insulin levels
- Expression levels of IFN-γ in tumor tissues
- Expression levels of TNF-α in tumor tissues
- 18-h fast
- Retro-orbital bleeding technique
- IgG-treated control mice
- Mouse IFN-γ ELISA kit
- Mouse TNF-α ELISA kit
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