Immunohistochemistry and Immunofluorescence of Myocardial Markers

IHC staining for CD86 (Abcam; ab53004; 1:1000); CD206 (Abcam; ab8918; 1:1000) and IF staining for Glut1 (E4S6I) (Rabbit mAb #73,015) and F4/80(C-7): sc-377009) was performed as previously described [26 (link)], with some modifications. Frozen sections were used; therefore, the original experiment's antigen retrieval phase was skipped, and myocardial slices were fixed in 4% formaldehyde for 15 min before staining. CD86 + and CD206 + were detected using a light microscope (Olumpus CX31) at X40 magnification. Also, Glut1 and F4/80 were detected using a fluorescence microscope at X60 magnification. They were then quantified using ImageJ (1.52a version; National Institute of Health USA).

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Ndzie Noah M.L., Mprah R., Wowui P.I., Adekunle A.O., Adu-Amankwaah J., Tan R., Gong Z., Li T., Fu L., Machuki J.O., Zhang S, & Sun H. (2024). CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner. Cell Communication and Signaling : CCS, 22, 166.

Publication 2024

ab53004 ab8918

Corresponding Organization :

Other organizations : Xuzhou Medical College

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Variable analysis

independent variables

IHC staining for CD86 (Abcam; ab53004; 1:1000)
IHC staining for CD206 (Abcam; ab8918; 1:1000)
IF staining for Glut1 (E4S6I) (Rabbit mAb #73,015)
IF staining for F4/80(C-7): sc-377009)

dependent variables

CD86 + and CD206 + quantification using a light microscope (Olumpus CX31) at X40 magnification
Glut1 and F4/80 quantification using a fluorescence microscope at X60 magnification

control variables

Frozen sections were used; therefore, the original experiment's antigen retrieval phase was skipped
Myocardial slices were fixed in 4% formaldehyde for 15 min before staining

controls

Positive and negative controls were not explicitly mentioned in the input.

Annotations

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