Comprehensive Western Blotting Protocol

Western blotting was conducted in accordance with previously described standard protocols [16 (link)]. Primary antibodies, including anti-Beta Actin (20536-1-AP, Proteintech, China), anti-α-SMA (14395-1-AP, Proteintech, China), anti-CD31 (11265-1-AP, Proteintech, China), anti-E-cadherin (20874-1-AP, Proteintech, China), anti-Vimentin (10366-1-AP, Proteintech, China), anti-ZEB1 (21544-1-AP, Proteintech, China), anti-HIF1α (20960-1-AP, Proteintech, China), anti-HIF1α-OH402 (ab72775, Abcam, USA), anti-HIF1α-OH564 (#3434, CST, USA), anti-PHD1 (12984-1-AP, Proteintech, China), anti-PHD2 (19886-1AP, Proteintech, China), and anti-PHD3 (18325-1-AP, Proteintech, China) were used. Secondary antibodies, consisting of goat anti-mouse and anti-rabbit antibodies conjugated with horseradish peroxidase (Proteintech, China), were utilized, and the blots were detected utilizing enhanced chemiluminescence (ECL) (P10300, NCM, China). Quantitative analysis of western blotting was performed using ImageJ.