Colony formation assay was performed following the method described previously [19 (link)]. In detail, 5,000 (A549 and SPC-A-1) or 20,000 (A549, 95D, H1299, SPC-A-1) cells per well were seeded and cultured in 24- or 6-well plates for 14 days. Then, cells were washed with 1 × PBS, fixed with 4% formaldehyde, stained with 0.1% crystal violet, and recorded by EOS 90D (Canon, Japan).
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