Immunoblot Analysis of Cell Lysates

Cell lysates were prepared for immunoblot analysis as previously described (Silva et al., 2014 (link); Silva and McMahon, 2014 (link)). Membranes were probed with the primary antibodies as described in Supplementary Table S3. Antigen-antibody complexes were detected using fluorescent goat anti-Rabbit or anti-Mouse IRDye 800 or goat anti-Mouse IRDye 680 secondary antibodies (LI-COR Biosciences) and visualized using a LI-COR Odyssey Classic infrared imaging system. Immunoblot data was analyzed using the Odyssey application software v3.0.30 software (LI-COR Biosciences) (Silva and McMahon, 2014 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Silva J.M., Deuker M.M., Baguley B.C, & McMahon M. (2017). PIK3CA-Mutated Melanoma Cells Rely on Cooperative Signaling through mTORC1/2 for Sustained Proliferation. Pigment cell & melanoma research, 30(3), 353-367.

Publication 2017

goat anti-Mouse IRDye 680 Odyssey Classic infrared imaging system

Anti antibodies Antibodies Antigen antibody complexes Cell Goat Immunoblot Irdye 800 Membranes Mouse Rabbit

Corresponding Organization : University of California, San Francisco

Other organizations : Cancer Society of New Zealand, University of Auckland

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression levels

control variables

Not explicitly mentioned

controls

Positive control: No explicit mention
Negative control: No explicit mention

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!