Purification and Detection of HEGF Protein

The extraction and detection methods for hegf employ in this study were identical to that reported previously [38 (link), 39 (link)]. His-tagged hegf protein was extracted from culture supernatant using a protein purification kit (Sangon Biotech, Shanghai). The protein electrophoresis procedure was identical to the previous publication [40 (link)]. SAM was extracted and measured using our previously established method [41 (link), 42 (link)].

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Cheng B., Yu K., Weng X., Liu Z., Huang X., Jiang Y., Zhang S., Wu S., Wang X, & Hu X. (2024). Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications. Microbial Cell Factories, 23, 55.

Publication 2024

protein purification kit

Corresponding Organization :

Other organizations : Jiangnan University, Guangxi Medical University, AgResearch, Massey University, State Key Laboratory of Food Science and Technology

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Variable analysis

independent variables

Extraction and detection methods for hegf

dependent variables

Presence and levels of hegf protein

control variables

Extraction and detection methods for hegf protein, which were identical to those reported previously [38, 39]
Protein electrophoresis procedure, which was identical to the previous publication [40]
SAM extraction and measurement, which used the previously established method [41, 42]

positive controls

Not specified

negative controls

Not specified

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