Spleen Total RNA Extraction and Sequencing

Total RNA was isolated from spleen using Trizol reagent. Then mRNA samples library construction and high-throughput sequencing were performed by Hangzhou KaiTai Biotechnology Co., Ltd. In brief, Polyadenylated RNA was enriched from total RNA. mRNA samples were fragmented into 300-nucleotide-long fragments using RNA Fragmentation Reagents (Ambion, AM8740) and used for library construction and performed high-throughput sequencing on Illumina Novaseq 6000.

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Liao C., Luo S., Liu X., Zhang L., Xie P., Zhou W., Lu Y., Zhong H., Zhang X., Xiong Z., Huang X., Mo G., Ma D, & Tang J. (2024). Siglec-F+ neutrophils in the spleen induce immunosuppression following acute infection. Theranostics, 14(6), 2589-2604.

Publication 2024

RNA Fragmentation Reagents Novaseq 6000

Corresponding Organization :

Other organizations : Guangdong Medical College, Chelsea and Westminster Hospital, Imperial College London, Children's Hospital of Zhejiang University

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Variable analysis

independent variables

Trizol reagent used for total RNA isolation from spleen

dependent variables

MRNA samples for library construction and high-throughput sequencing

control variables

Polyadenylated RNA enrichment from total RNA
MRNA fragmentation into 300-nucleotide-long fragments using RNA Fragmentation Reagents (Ambion, AM8740)

controls

Positive control: Not specified
Negative control: Not specified

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