SDS-PAGE and Immunoblotting was performed as described previously (Lee, 2012 (link)).
For immunoblotting analysis the following commercial primary antibodies were used
in this study: anti-Cyclin D1 (#2922) and anti-Cyclin D3 (# 2936) were purchased
from Cell Signaling Technology (Danvers, MA, USA); anti-Cyclin B1 (H-433),
anti-p53 (SC-126), and anti-ERK1 (SC-94) were purchased from Santa Cruz
Biotechnology (Santa Cruz, CA, USA); anti-p21 (ab7960) was purchased from Abcam
(Cambridge, MA, USA). The primary antibodies were visualized with goat
anti-rabbit (SC-2004, Santa Cruz Biotechnology, Santa Cruz) or goat anti- mouse
(SC-2005, Santa Cruz Biotechnology) antibodies conjugated with horseradish
peroxidase and an enhanced chemiluminescence (ECL) detection system.