Intranasal Allergen Challenge in Pla2g5-Null Mice

C57/BL6 Wt and Pla2g5-null mice^{48 (link), 49 (link)} (9–12 wk-old males) received 25 μg of Alternaria alternata extract (Greer Laboratories, Lenoir, NC) in 20 uL of PBS or PBS alone intranasally (i.n.) on days 0,3,6 and 9 and euthanized 18h later^{1 (link)} or a single dose of 100 μg and were euthanized after 1h or 3h². Alternatively, Wt and Pla2g5-null naïve mice were given mouse rIL-33 (R&D Systems, Minneapolis, MN) i.n. 100 ng/dose on days 0,3,6 and 9 with or without LA (132 nM)^{28 (link)} OA (106 nM) or AA (99 nM), and mice were euthanized 18h after the last dose. All animal experiments were approved by the Animal Care and Use Committee of the Dana-Farber Cancer Institute (Boston, MA).