3T MRI Brain Lesion Quantification Protocol

Participants underwent 3T brain MRI on a Philips Achieva 3T scanner with an 8-channel SENSE head coil. The scan protocol included 3D T1-weighted gradient echo, 3D T2-weighted turbo spin echo, 3D T2*-weighted gradient echo, and 3D fluid-attenuated inversion recovery (FLAIR) scan (sequence details eTable 1, links.lww.com/WNL/D397). Lacunes (on T1-weighted, T2-weighted, and FLAIR scan) were manually counted, and microbleed counting (on T2*-weighted scans) was supported by semiautomated rating software.^{16 (link)} Ratings were performed by 2 trained raters (HvdB and NAW who had a good interrater reliability of 0.8 or more and resolved discrepancies in a consensus meeting) according to the STRIVE-1 criteria^{17 (link)} (note: the criteria for these particular lesions are the same in the updated STRIVE-2).^{18 (link)} Segmentations of WMH, lacunes, intracranial volume, and total brain volume were acquired as previously published.^{13 (link)}