Mice were anesthetized by intraperitoneal injection with Ketamine/Xylazine (100 mg/kg and 10 mg/kg) and bleomycin (2.5 units/kg) (Sigma-Aldrich, St Louis, MO) in phosphate-buffered saline (PBS) or vehicle (PBS) alone was administered intratracheally (Sueblinvong et al., 2014a (link)). At 7 or 14 days after bleomycin injection, the lungs were harvested and processed for Western blot analysis.
Protocol detail
Bleomycin-Induced Lung Injury in Mice
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Emory University
Other organizations : Atlanta VA Medical Center
Top 5 similar protocols
Variable analysis
independent variables
- Bleomycin (2.5 units/kg) in phosphate-buffered saline (PBS)
- Vehicle (PBS) alone
- Protein expression (measured by Western blot analysis)
- Anesthesia (Ketamine/Xylazine at 100 mg/kg and 10 mg/kg)
- Positive control: Mice receiving bleomycin
- Negative control: Mice receiving vehicle (PBS) alone
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!