Wild-type C57BL/6, caspase-1−/− and NLRP3−/− mice were obtained from The Jackson Laboratory, USA [8 (link),13 (link)]. The knockout mice were crossed onto the C57BL/6 background for at least nine generations. Pregnant mice were injected with EVs at days 10.5 and 11.5 p.c., as described earlier [8 (link),14 (link)]. Anakinra or aspirin was injected intraperitoneally 30 min prior to each EV injection [8 (link),15 (link)]. All animal experiments were conducted following standards and procedures approved by the local Animal Care and Use Committee (Landesverwaltungsamt, Halle, Germany).
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