For internalization
assays, the cells were incubated for 24 h, washed to remove membrane-bound
AuNPs using 0.1 M glycine HCl at pH 2.5, and lysed with 0.1 M NaOH
as previously described.36 (link) A Nuclei EZ
Prep Nuclei Isolation kit (Sigma-Aldrich #NUC101-1KT) was used to
determine the subcellular distribution of AuNP constructs. The cytosol
and nuclei were isolated according to the manufacturer’s protocol.
After separation of fractions, the samples were counted in a Wizard3”
automatic gamma-counter.
assays, the cells were incubated for 24 h, washed to remove membrane-bound
AuNPs using 0.1 M glycine HCl at pH 2.5, and lysed with 0.1 M NaOH
as previously described.36 (link) A Nuclei EZ
Prep Nuclei Isolation kit (Sigma-Aldrich #NUC101-1KT) was used to
determine the subcellular distribution of AuNP constructs. The cytosol
and nuclei were isolated according to the manufacturer’s protocol.
After separation of fractions, the samples were counted in a Wizard3”
automatic gamma-counter.
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