Protocol detail

Illumina Sequencing of S. pombe and Mouse cDNA

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We sequenced all the cDNA libraries with an Illumina Genome Analyzer IIx. We pooled the four S. pombe libraries together with four other indexed libraries and sequenced them using eight lanes of 76 nucleotide paired reads. We sequenced the mouse library using two lanes of 76 nucleotide paired reads.

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Grabherr M.G., Haas B.J., Yassour M., Levin J.Z., Thompson D.A., Amit I., Adiconis X., Fan L., Raychowdhury R., Zeng Q., Chen Z., Mauceli E., Hacohen N., Gnirke A., Rhind N., di Palma F., Birren B.W., Nusbaum C., Lindblad-Toh K., Friedman N, & Regev A. (2011). Trinity: reconstructing a full-length transcriptome without a genome from RNA-Seq data. Nature biotechnology, 29(7), 644-652.

Publication 2011

Cdna Cdna libraries Genome Genome libraries Library Mouse Nucleotide

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Protocol cited in 616 other protocols

Variable analysis

independent variables

Sequencing platform: Illumina Genome Analyzer IIx

dependent variables

Sequencing output of cDNA libraries

control variables

Sequencing read length: 76 nucleotides
Number of lanes used for sequencing: 8 lanes for pooled S. pombe libraries, 2 lanes for mouse library

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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