Nutrient Supply Analysis in Cultivation Chambers

Computational fluid dynamics simulations (CFD) were performed to analyze nutrient supply inside the cultivation chamber designs as described before^{13 (link),27 (link)}. For the CFD simulations COMSOL Multiphysics Version 5.6 (COMSOL AB, Sweden) was applied. Glucose concentration profiles during MSCC, assuming a steady state with 181 cells inside the chamber with a constant glucose uptake rate of 3800 nmol per 10⁶ cells and day, were simulated under consideration of the respective cultivation chamber geometry. Cellular uptake was assumed to be homogeneously distributed over the whole cultivation chamber. Glucose concentration inside the supply channels was set to 45 mol m⁻³.