Arabidopsis thaliana (L.) Heynh. var. Columbia (Col-0) and var. Landsberg (erecta mutant) (Ler) were used as wild-type strains. The sub-1 mutant was described previously [25] (link). Plants were grown in a greenhouse under Philips SON-T Plus 400 Watt fluorescent bulbs on a long day cycle (16 hrs light). Dry seeds were sown on soil (Patzer Einheitserde, extra-gesiebt, Typ T, Patzer GmbH & Co. KG, Sinntal-Jossa, Germany) overlying perlite, stratified for 4 days at 4°C and then placed in the greenhouse. Plant trays were covered for 7–8 days to increase humidity and support equal germination. Ler seeds mutagenized with ethylmethane sulfonate (EMS) were obtained from Lehle Seeds (Round Rock, TX, USA). 60'000 M2 plants, corresponding to about 7'500 M1 plants, were screened for plants exhibiting a sub-like phenotype. All sub-like mutants described in this paper were outcrossed three times to Ler prior to further analysis. Two qky T-DNA insertion mutants (line SALK_140123 and SALK_043901) [112] (link) were obtained from the ABRC (http://www.arabidopsis.org). The GL2::GUS line [33] (link) in Ler was crossed into slm mutants for analysis of root hair specification.
Free full text: Click here