On the next day after last MSs or vehicle administration, all survived animals were randomly divided into the following groups (Figure 1):
Healthy animals (INT)—sham surgery has been performed in these animals which received vehicle only;
CTEPH—sham surgery has been performed in these animals with CTEPH;
CTEPH + Sympathetic Denervation (CTEPH + SD)—the animals with CTEPH were subjected to unilateral surgical sympathetic denervation;
CTEPH + Vagal Denervation (CTEPH + VD)—the animals with CTEPH were subjected to unilateral surgical vagal denervation;
CTEPH + Pyridostigmine (CTEPH + PS)—the animals with CTEPH were treated with reversible cholinesterase inhibitor pyridostigmine.
Six weeks after surgery, echocardiography was performed in all animals. Then, hemodynamic measurements were carried out in anesthetized animals, followed by blood sampling, euthanasia and heart and lung harvesting for histological and molecular biology studies.
Karpov A.A., Vachrushev N.S., Shilenko L.A., Smirnov S.S., Bunenkov N.S., Butskih M.G., Chervaev A.K., Vaulina D.D., Ivkin D.Y., Moiseeva O.M, & Galagudza M.M. (2023). Sympathetic Denervation and Pharmacological Stimulation of Parasympathetic Nervous System Prevent Pulmonary Vascular Bed Remodeling in Rat Model of Chronic Thromboembolic Pulmonary Hypertension. Journal of Cardiovascular Development and Disease, 10(2), 40.
Other organizations :
Ministry of Health of the Russian Federation, Institute of Experimental Medicine, Federal Almazov North-West Medical Research Centre, First Pavlov State Medical University of St. Petersburg
Healthy animals (INT) - sham surgery with vehicle only
CTEPH - sham surgery with CTEPH
controls
Positive Control: Healthy animals (INT) - sham surgery with vehicle only
Negative Control: CTEPH - sham surgery with CTEPH
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