Sampling and Filtration of A. sanguinea Bloom

Samples were collected along the Xiamen coast at sites within (A1; N 24°35′53.40′′, E 118°9′29.67′′) or near (H1; N 24°36′56.31′′, E 118°9′15.92′′) the A. sanguinea bloom (Fig. 1), during the summer of 2011. Samples were collected each day from 31 July to 5 August as described previously25 (link). Briefly, three replicate water samples (20 l) were collected from each site and filtered through 5-μm diameter pore-size filters (Millipore, US) to remove A. sanguinea and other large algae. The total free-living bacteria in the filtrate were then collected with 0.22-μm filters (Millipore, US) and stored at −70 °C until analysis.

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Yang C., Li Y., Zhou Y., Lei X., Zheng W., Tian Y., Van Nostrand J.D., He Z., Wu L., Zhou J, & Zheng T. (2016). A comprehensive insight into functional profiles of free-living microbial community responses to a toxic Akashiwo sanguinea bloom. Scientific Reports, 6, 34645.

Publication 2016

pore-size filters 0.22-μm filters

Bacteria Replicate Size

Corresponding Organization :

Other organizations : Xiamen University, Southwest University, Henan Normal University, University of Oklahoma, Tsinghua University, Lawrence Berkeley National Laboratory

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Variable analysis

independent variables

Sampling location (A1 and H1)

dependent variables

Total free-living bacteria in the filtrate

control variables

Sampling date (31 July to 5 August)
Sample volume (20 l)
Filter pore size (5 μm and 0.22 μm)
Storage temperature (-70 °C)

controls

No positive or negative controls were explicitly mentioned in the protocol.

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