Murine Macrophage Isolation and Hypoxia Experiments

All procedures involving animals were approved by the UCSD Animal Care Committee, which serves to ensure that all federal guidelines concerning animal experimentation are met. Floxed Syk mice and lysozyme M (LysM) Cre recombinase transgenic mice were purchased from Jackson laboratories. Integrin α4Y991A mice and normal littermates in C57BL/6J genetic background have been described before (16 (link),21 (link)). BMDMs were isolated as described previously (16 (link)). For hypoxia experiments, MΘs were placed in a modulator incubator chamber (Billups-Rothenberg) as described before (16 (link)).

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Joshi S., Liu K.X., Zulcic M., Singh A.R., Skola D., Glass C.K., Sanders P.D., Sharabi A.B., Pham T.V., Tamayo P., Shiang D., Dinh H.Q., Hedrick C.C., Morales G.A., Garlich J.R, & Durden D.L. (2020). Macrophage Syk-PI3Kγ inhibits anti-tumor immunity: SRX3207, a novel dual Syk-PI3K inhibitory chemotype relieves tumor immunosuppression. Molecular cancer therapeutics, 19(3), 755-764.

Publication 2020

modulator incubator chamber

Animal care committee Animals Cre recombinase Genetic background Hypoxia Integrin Lysozyme Mice Transgenic mice

Corresponding Organization :

Other organizations : University of California, San Diego, La Jolla Institute For Allergy & Immunology, SignalRx Pharmaceuticals (United States)

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Variable analysis

independent variables

Genotypes of mice (Floxed Syk mice, LysM Cre recombinase transgenic mice, Integrin α4Y991A mice, and normal littermates)
Oxygen levels (normoxia vs. hypoxia)

dependent variables

Phenotypes of bone marrow-derived macrophages (BMDMs)

control variables

C57BL/6J genetic background of Integrin α4Y991A mice and normal littermates
Procedures involving animals were approved by the UCSD Animal Care Committee to ensure compliance with federal guidelines

controls

Normal littermates as a negative control for the Integrin α4Y991A mice

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