Protocol detail

Targeted Immunomodulation in Lung Cancer

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The anti-NK1.1 depletion experiment was performed in Kras/p53 driven lung cancer model, while IFNγ and CCRL2 blocking in the LG cell line lung metastasis model. Mice were treated at day -1 intraperitoneally with 100 μg of anti-NK1.1 (clone PK136) or isotype control (clone C1.18.4) and anti-CCRL2, generated in the lab (Otero et al., 2010 (link)), or isotype control (clone MPC-11); and 200 μg of anti-IFNγ (clone XMG1.2) or isotype control (IgG1k HRPN) (all from BioXCell). Mice were then treated with 100 μg once (anti-NK1.1) or three times (anti-CCRL2; anti-IFNγ) a week for the entire duration of the experiment.

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Del Prete A., Sozio F., Schioppa T., Ponzetta A., Vermi W., Calza S., Bugatti M., Salvi V., Bernardini G., Benvenuti F., Vecchi A., Bottazzi B., Mantovani A, & Sozzani S. (2019). The atypical receptor CCRL2 is essential for NK cell-dependent resistance against lung cancer. Cancer immunology research, 7(11), 1775-1788.

Publication 2019

anti-NK1.1 (clone PK136) anti-IFNγ (clone XMG1.2)

Ccrl2 Cell Clone Ifnγ Isotype Kras Lung Lung cancer Metastasis Mice

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Variable analysis

independent variables

Anti-NK1.1 depletion
IFNγ blocking
CCRL2 blocking

dependent variables

Lung cancer model
Lung metastasis model

control variables

Isotype control for anti-NK1.1
Isotype control for anti-CCRL2
Isotype control for anti-IFNγ

controls

Positive control: Isotype control (clone C1.18.4) for anti-NK1.1
Positive control: Isotype control (clone MPC-11) for anti-CCRL2
Positive control: Isotype control (IgG1k HRPN) for anti-IFNγ
Negative control: Not explicitly mentioned

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