Immunohistochemical Staining of WHSC1 and P4HB

IHC staining was performed using anti-WHSC1 (1:100, ab223694; Abcam, Cambridge, UK) and anti-P4HB (1:200, ab2792; Abcam, Cambridge, UK) antibodies. The scoring system for IHC staining was applied as previously described.17 (link)

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Dai J., Jiang L., Qiu L., Shao Y., Shi P, & Li J. (2020). WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling. OncoTargets and therapy, 13, 7033-7044.

Publication 2020

ab223694 ab2792

Antibodies Whsc1

Corresponding Organization :

Other organizations : Nanjing Medical University, The First People’s Hospital of Lianyungang, Xian Yang Central Hospital

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Variable analysis

independent variables

Anti-WHSC1 antibody (1:100, ab223694; Abcam, Cambridge, UK)
Anti-P4HB antibody (1:200, ab2792; Abcam, Cambridge, UK)

dependent variables

IHC staining score

control variables

Scoring system for IHC staining, as previously described in the referenced publication

controls

Positive control
Negative control

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