Isolation of Rat Lung and Human Mesothelial Cells

Rat lung primary cells were prepared from the lung of a young adult (<1 year-old) Sprague Dawley rat from the Office of Laboratory Animal Care (University of California at Berkeley) following published procedures [14 (link)]. The neoplastic F10 rat clone was derived from a culture of SV40 virus-infected rat lung cells as described by us previously [14 (link)]. Primary human mesothelial cells were prepared and infected with SV40 virus as described by Bocchetta et al. [98 (link)]. The cells were grown on plastic culture dishes (Corning Falcon, NY, USA) in RPMI 1640 medium (Corning Cellgro, MA, USA) supplemented with 3–5% fetal calf serum, 3–5% calf serum (Hyclone, UT, USA), 1% Antibiotic Antimycotic and 1% Nyastin (Sigma Co., St Louis, MI, USA) following published procedures [14 (link),71 (link)].