Measurement of Cardiomyocyte Function

Isolated LV myocytes were placed in a bath located on the stage of an Axiovert (Zeiss), IX51 and IX71 (Olympus) inverted microscopes for measurements of contractility, Ca²⁺ transients and patch-clamp studies.^{24 (link)–27 (link)} Experiments were performed at room temperature.

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Signore S., Sorrentino A., Ferreira-Martins J., Kannappan R., Shafaie M., Ben F.D., Isobe K., Arranto C., Wybieralska E., Webster A., Sanada F., Ogórek B., Zheng H., Liu X., del Monte F., D’Alessandro D.A., Wunimenghe O., Michler R.E., Hosoda T., Goichberg P., Leri A., Kajstura J., Anversa P, & Rota M. (2013). Inositol 1, 4, 5-Triphosphate Receptors and Human Left Ventricular Myocytes. Circulation, 128(12), 10.1161/CIRCULATIONAHA.113.002764.

Publication 2013

Bath Contractility Microscopes Myocytes Transients

Corresponding Organization :

Other organizations : Brigham and Women's Hospital, Beth Israel Deaconess Medical Center, Harvard University, Albert Einstein College of Medicine, Montefiore Medical Center

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Protocol cited in 15 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Contractility
Ca2+ transients
Patch-clamp measurements

control variables

Temperature (room temperature)

controls

No positive or negative controls were explicitly mentioned in the provided text.

Annotations

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