Antibacterial Evaluation of Essential Oils

The following bacterial species were used: Bacillus subtilis (ATCC 10707), Enterobacter cloacae (human isolate), Escherichia coli (ATCC 0157:H7), Micrococcus flavus (ATCC 9341),Proteus mirabilis (human isolate), Pseudomonas aeruginosa (ATCC 27853), Salmonella enteritidis (ATCC 13076), S. epidermidis (ATCC 12228) S. typhimurium (ATCC 13311) Staphylococcus aureus (ATCC 25923). The antibacterial assays were carried out by the disc-diffusion [25 (link)] and microdilution method [26 ,27 (link),28 (link)] in order to determine the antibacterial activity of oils and their components against the human pathogenic bacteria. The bacterial suspensions were adjusted with sterile saline to a concentration of 1.0 × 10⁵ CFU/mL. The inocula were prepared daily and stored at +4 °C until use. Dilutions of the inocula were cultured on solid medium to verify the absence of contamination and to check the validity of the inoculum.

Free full text: Click here

Soković M., Glamočlija J., Marin P.D., Brkić D, & van Griensven L.J. (2010). Antibacterial Effects of the Essential Oils of Commonly Consumed Medicinal Herbs Using an In Vitro Model. Molecules, 15(11), 7532-7546.

Publication 2010

Antibacterial Assays Bacillus subtilis Bacterial Cultured medium Diffusion Dilutions Enterobacter cloacae Escherichia coli Human Micrococcus flavus Oils Pathogenic Proteus mirabilis Pseudomonas aeruginosa S epidermidis Saline Salmonella enteritidis Staphylococcus aureus Sterile

Corresponding Organization : Wageningen University & Research

Other organizations : University of Belgrade

Top 5 similar protocols

Protocol cited in 24 other protocols

Variable analysis

independent variables

Bacillus subtilis (ATCC 10707)
Enterobacter cloacae (human isolate)
Escherichia coli (ATCC 0157:H7)
Micrococcus flavus (ATCC 9341)
Proteus mirabilis (human isolate)
Pseudomonas aeruginosa (ATCC 27853)
Salmonella enteritidis (ATCC 13076)
Staphylococcus epidermidis (ATCC 12228)
Salmonella typhimurium (ATCC 13311)
Staphylococcus aureus (ATCC 25923)

dependent variables

Antibacterial activity of oils and their components

control variables

Bacterial suspensions were adjusted with sterile saline to a concentration of 1.0 × 10^5 CFU/mL
The inocula were prepared daily and stored at +4 °C until use
Dilutions of the inocula were cultured on solid medium to verify the absence of contamination and to check the validity of the inoculum

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!